Generator

Part:BBa_K145205

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-20)

OUTPUT GFP under TetR pomoter

OUTPUT GFP with an LVA tag under the control of the TetR pomoter. This means repression if the Tet repressor is present and activation when (anhydro)tetracyclin is added. If no TetR or aTc is present, there will also be transcription of GFP-LVA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


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Categories
Parameters
None